Yuan Pang
Yuan Pang Ph.D
Assistant Professor
Bio-manufacturing center, Tsinghua UniversityBiomanufacturing Center Dept. of Mechanical Engineering, Tsinghua University
Email: pangyuan31@tsinghua.edu.cn
Biography:
Dr. Pang received Ph.D. in Bio-engineering from the University of Tokyo in 2013 and did her postdoc fellowship at the Institute of Industry and Science (Prof. Yasuyuki Sakai’s Lab), University of Tokyo in 2013-2015. She started her work as an Assistant Professor at Bio-manufacturing center in Tsinghua University since 2016. During her research carrier, she got several scientific awards such as young scientific award of International Society of Biomaterials, publication awards of Tissue Engineering and Regenerative Medicine International Society- Asia Pacific. She recently became the committee member of Chinese Society of Toxicity Testing and Alternatives, CEMS.
Her current research interests focus on construction of in vitro functional tissue models through bio-printing, includes: 1) 3D printed tumor model and its application in cancer metastasis study; 2) 3D printed implantable tissue substitutes with drug release functionality; 3) vascularized micro-organ fabrication; 4) 3D printed graphene oxide bio-electrode.
Abstract:
Organization of liver organoid using Raschig ring-like micro-scaffolds and triple co-culture toward modular assembly-based scalable liver tissue engineering
Addressing the remaining issues of fragile structure and insufficient mass transfer in modular assembly-based tissue engineering, a Raschig ring-like micro-scaffold was proposed and fabricated using poly-ε-caprolactone with 60% porosity and 11.4 mm2 efficient surface area for cell loading. By investigation on the inoculation process, the extracellular matrix was found necessary to establish the hierarchical co-culture of Hep G2 and endothelial cells, and the triple co-culture with fibroblast (Swiss 3T3 cells) were recognized most efficient for attempting to higher cell attachment, proliferation and hepatic function. The equipped intersecting hollow channels in the micro-scaffold functioned as flow paths after randomly packed into a bioreactor for perfusion culture. The hollow structure promoted the mass transfer to the immobilized cells, as turned out the advanced albumin production and well maintained cellular viability. In vivo-comparable cell density was obtained in the perfused construct with well-preserved rigid structure, which suggested the great potential of current micro-scaffolds as modular tissues towards scaling up in the organization of liver organoid.
Assistant Professor
Bio-manufacturing center, Tsinghua UniversityBiomanufacturing Center Dept. of Mechanical Engineering, Tsinghua University
Email: pangyuan31@tsinghua.edu.cn
Biography:
Dr. Pang received Ph.D. in Bio-engineering from the University of Tokyo in 2013 and did her postdoc fellowship at the Institute of Industry and Science (Prof. Yasuyuki Sakai’s Lab), University of Tokyo in 2013-2015. She started her work as an Assistant Professor at Bio-manufacturing center in Tsinghua University since 2016. During her research carrier, she got several scientific awards such as young scientific award of International Society of Biomaterials, publication awards of Tissue Engineering and Regenerative Medicine International Society- Asia Pacific. She recently became the committee member of Chinese Society of Toxicity Testing and Alternatives, CEMS.
Her current research interests focus on construction of in vitro functional tissue models through bio-printing, includes: 1) 3D printed tumor model and its application in cancer metastasis study; 2) 3D printed implantable tissue substitutes with drug release functionality; 3) vascularized micro-organ fabrication; 4) 3D printed graphene oxide bio-electrode.
Abstract:
Organization of liver organoid using Raschig ring-like micro-scaffolds and triple co-culture toward modular assembly-based scalable liver tissue engineering
Addressing the remaining issues of fragile structure and insufficient mass transfer in modular assembly-based tissue engineering, a Raschig ring-like micro-scaffold was proposed and fabricated using poly-ε-caprolactone with 60% porosity and 11.4 mm2 efficient surface area for cell loading. By investigation on the inoculation process, the extracellular matrix was found necessary to establish the hierarchical co-culture of Hep G2 and endothelial cells, and the triple co-culture with fibroblast (Swiss 3T3 cells) were recognized most efficient for attempting to higher cell attachment, proliferation and hepatic function. The equipped intersecting hollow channels in the micro-scaffold functioned as flow paths after randomly packed into a bioreactor for perfusion culture. The hollow structure promoted the mass transfer to the immobilized cells, as turned out the advanced albumin production and well maintained cellular viability. In vivo-comparable cell density was obtained in the perfused construct with well-preserved rigid structure, which suggested the great potential of current micro-scaffolds as modular tissues towards scaling up in the organization of liver organoid.