Guifeng Zhang
Guifeng Zhang, Professor
State Key Laboratory of Biochemical Engineering
Institute of Process Engineering, Chinese Academy of Sciences
No.1 North 2nd Street, Zhongguancun, Haidian District
Beijing100190, China
E-mail: gfzhang@ipe.ac.cn
Research interests
[1] Preparation of collagen-based biomaterials and its application in bio implant and tissue engineering.
[2] Role of collagen in the regulation of cell migration and differentiation.
[3] Effect of proline hydroxylation in collagen on protein-protein interaction.
Recent Publications
[1] Gongze Peng, Saina Li, Qing Peng, Yang Li, Jun Weng, Zhidong Jia, Xiongxin Lei, Guifeng Zhang, Yi Gao. Immobilization of native type I collagen on polypropylene fabrics as a substrate for HepG2 cell culture. Journal of Biomaterials Applications, 2017, 32 (1): 93-103.
[2] Huimei Wang, Shulan Han, Lianyan Wang, Tingyuan Yang, Guifeng Zhang, LianYu, Yue Zhao. Dual-function baicalin and baicalin-loaded poly(lactic-co-glycolic acid) nanoparticles: Immune activation of dendritic cells and arrest of the melanoma cell cycle at the G2/M phase. Particuology, 2018, 37: 64-71.
[3] Qing Li, Tong Wang, Guifeng Zhang, Xin Yu, Jing Zhang, Gang Zhou, Zhi- hui Tang. A comparative evaluation of the mechanical properties of two calcium phosphate/collagen composite materials and their osteogenic effects on adipose-derived stem cells. Stem Cells International, 2016, 395:1-12.
[4] Tong Wang, Qing Li, Gui-feng Zhang, Gang Zhou, Xin Yu, Xiu-mei Wang, Zhihui Tang. Comparative evaluation of a biomimic collagen/hydroxyapatite/β-tricaleium phosphate scaffold in alveolarridge preservation with Bio-Oss Collagen. Front. Mater. Sci. 2016, 10(2): 122–133
Ongoing Projects
[1] Preparation of collagen-coated materials and its application as bioartificial liver support.
[2] Preparation of polysacchride-protein composite to be used as haemostatic materials.
[3] Decellularization of extracellular matrix of liver to be used as cell scaffold.
Abstract:
Collagen based biomaterials and its application in biopriting
The collagen family is one of the most abundant groups of proteins as principal protein component of all connective tissues. Due to its vital role in tissue, collagen is regarded as one of the most useful biomaterials, either as native, unmodified tissue grafts or as manufactured products for use in various medical areas. The excellent biocompatibility and safety due to its biological characteristics, such as biodegradability and weak antigenicity, made collagen the primary resource in medical applications. Collagen stability under different conditions is different, based on which, we established a collagen extraction process. Collagen type I and II was prepared with industrial scale. A method for collagen type identification and characterization was developed with marker peptide as an index using HPLC-MS/MS.
Among the existing collagens, collagen type I is the most abundant and also the most important for use as a biomaterial. However, The properties of collagen change with embryonic growth and development, for example, embryonic dermal collagen contains a high proportion of type III collagen but is predominantly type I in the adult, similarly endochondral ossification involves the replacement of type II collagen by type I. Theoretically, preparation of articular cartilage of cell scaffold to be used for articular should use type II as raw materials. Thus, we investigated the difference of cell behaviors on scaffold prepared with type I and II. Cell migration and differentiation is associated with ECM formation and degradation. The interaction between collagen and fibronectin, further with integrin play vital role in regulating cell behaviors. Thus, collagen and acellular matrix were used as matrix for bio printing. The protein map were profiled using proteomic method.
State Key Laboratory of Biochemical Engineering
Institute of Process Engineering, Chinese Academy of Sciences
No.1 North 2nd Street, Zhongguancun, Haidian District
Beijing100190, China
E-mail: gfzhang@ipe.ac.cn
Research interests
[1] Preparation of collagen-based biomaterials and its application in bio implant and tissue engineering.
[2] Role of collagen in the regulation of cell migration and differentiation.
[3] Effect of proline hydroxylation in collagen on protein-protein interaction.
Recent Publications
[1] Gongze Peng, Saina Li, Qing Peng, Yang Li, Jun Weng, Zhidong Jia, Xiongxin Lei, Guifeng Zhang, Yi Gao. Immobilization of native type I collagen on polypropylene fabrics as a substrate for HepG2 cell culture. Journal of Biomaterials Applications, 2017, 32 (1): 93-103.
[2] Huimei Wang, Shulan Han, Lianyan Wang, Tingyuan Yang, Guifeng Zhang, LianYu, Yue Zhao. Dual-function baicalin and baicalin-loaded poly(lactic-co-glycolic acid) nanoparticles: Immune activation of dendritic cells and arrest of the melanoma cell cycle at the G2/M phase. Particuology, 2018, 37: 64-71.
[3] Qing Li, Tong Wang, Guifeng Zhang, Xin Yu, Jing Zhang, Gang Zhou, Zhi- hui Tang. A comparative evaluation of the mechanical properties of two calcium phosphate/collagen composite materials and their osteogenic effects on adipose-derived stem cells. Stem Cells International, 2016, 395:1-12.
[4] Tong Wang, Qing Li, Gui-feng Zhang, Gang Zhou, Xin Yu, Xiu-mei Wang, Zhihui Tang. Comparative evaluation of a biomimic collagen/hydroxyapatite/β-tricaleium phosphate scaffold in alveolarridge preservation with Bio-Oss Collagen. Front. Mater. Sci. 2016, 10(2): 122–133
Ongoing Projects
[1] Preparation of collagen-coated materials and its application as bioartificial liver support.
[2] Preparation of polysacchride-protein composite to be used as haemostatic materials.
[3] Decellularization of extracellular matrix of liver to be used as cell scaffold.
Abstract:
Collagen based biomaterials and its application in biopriting
The collagen family is one of the most abundant groups of proteins as principal protein component of all connective tissues. Due to its vital role in tissue, collagen is regarded as one of the most useful biomaterials, either as native, unmodified tissue grafts or as manufactured products for use in various medical areas. The excellent biocompatibility and safety due to its biological characteristics, such as biodegradability and weak antigenicity, made collagen the primary resource in medical applications. Collagen stability under different conditions is different, based on which, we established a collagen extraction process. Collagen type I and II was prepared with industrial scale. A method for collagen type identification and characterization was developed with marker peptide as an index using HPLC-MS/MS.
Among the existing collagens, collagen type I is the most abundant and also the most important for use as a biomaterial. However, The properties of collagen change with embryonic growth and development, for example, embryonic dermal collagen contains a high proportion of type III collagen but is predominantly type I in the adult, similarly endochondral ossification involves the replacement of type II collagen by type I. Theoretically, preparation of articular cartilage of cell scaffold to be used for articular should use type II as raw materials. Thus, we investigated the difference of cell behaviors on scaffold prepared with type I and II. Cell migration and differentiation is associated with ECM formation and degradation. The interaction between collagen and fibronectin, further with integrin play vital role in regulating cell behaviors. Thus, collagen and acellular matrix were used as matrix for bio printing. The protein map were profiled using proteomic method.