Language: 简体中文 English
Jong-Young Kwak
Jong-Young Kwak, MD/PhD
Department of Pharmacology & Immune Network Pioneer Research Center,
Ajou University School of Medicine,
164, World cup-ro, Yeongtong-gu, Suwon 443-380,
T: +82-31-219-5064
F: +82-31-219-5069
M: +82-10-8514-7487

Dr. Kwak is professor at Department of Pharmacology, Ajou University School of Medicine, Korea. He is currently the Director of Immune-network Pioneer Research Center, Ajou University Medical Center. He became a vice-president of Korean Socie-ty of Biochemistry and Molecular Biology in 2015 and is a Doctor Honoris Causa in Russian Academy of Science since 2012. He completed his doctorate in Medical Bio-chemistry with neutrophil activation and signal transduction pathways at the Pusan National University, Korea in 1991. After his study of activation of neutrophils in Emory University as a post doctorate, he directed his research to dendritic cell analy-sis. Current research topic in his laboratory is immunogenic responses in 3D culture of immune cells and tissues. He is CEO of venture company, Nanofaentech Inc. in Korea.

Three Dimensional Monolayer Culture of Epithelial Cells on Electrospun Poly(vinyl alcohol) Nanofibrous Membrane

Adherence of epithelial cells cultured on traditional two-dimensional (2D) culture dish may induce aberrant cell functions, including epithelial-mesenchymal transition. In this study, three-dimensional (3D) culture system of epithelial cells was developed to maintain long-term survival, adherence, and function of cultured epithelial cells. Poly(vinyl alcohol) (PVA) is one of the most prevalent and versatile synthetic poly-mers abundantly used in tissue engineering as biomaterials, but high hydrophilicity of PVA results in water soluble and poor cell adhesion. The novel combination of PVA/polyacrylic acid/glutaraldehyde crosslinked with combination of heat, HCl, and dimethylformamide as an electrospun membrane showed promising characteristics of water stability and cell adhesion. In addition, peptides, which are derived from do-mains of cell binding in extracellular matrix proteins, such as fibronectin, laminin, and collagen, could be blended in PVA nanofibers. When mouse primary hepatocytes and lung epithelial cell line, MLE-12 cells were cultured on PVA nanofiber membrane, these cells adhered to the cross-linked PVA/PAA/GA nanofiber membrane but formed cell aggregate and discoidal shaped spheroid. In comparison, the epithelial cells adhered to and formed monolayer on cell-adhesive peptide-blended PVA nano-fibers. The expression of E-cadherin in culture cells and tight junction were main-tained during culture time. Therefore, epithelial cells cultured on PVA nanofiber membrane grow three dimensionally in monolayer and maintain their functions for long time.

Figure 1. Culture of primary hepatocytes for 5 days on PVA and RGD-PVA mem-brane

  • DAYS
Key Dates
   Deadline for Submission of Abstract:
  October 31, 2018
   Notification of abstract acceptance:
   November 15, 2018