Yasuyuki SAKAI
Yasuyuki SAKAI, PhD
Professor
Department of Chemical System Engineering and Department of Bioengineering, Graduate School of Engineering, University of Tokyo
Room 5A02, Eng. Build. No 3, Hongo 7-3-1, City of Bunkyo, Tokyo 113-8656, Japan
TEL/FAX, +81-3-5841-7073; E-MAIL, sakaiyasu@chemsys.t.u-tokyo.ac.jp
International Research Center on Integrative Biomedical Systems (CIBiS)
Institute of Industrial Science, University of Tokyo, Japan
http://orgbiosys.t.u-tokyo.ac.jp/sakai/index.php?lang=en&page=top
Biography:
Dr. Sakai is the current president of JSAAE (2017-2018) and a professor at Department of Chemical System Engineering, Graduate School of Engineering, University of Tokyo (UTokyo), Japan. He also belongs to Department of Bioengineering. He received Ph.D. in chemical engineering from UTokyo in 1993 and stated his work at Institute of Industrial Science (IIS), UTokyo. In 1997-1998, he stayed in University of Rochester as a visiting scientist. In 2003-2008, he worked as an associate professor of Regenerative Medical Engineering Laboratory, Graduate School of Medicine, UTokyo. He returned to IIS as a professor and then moved to the current position in 2015. During his research carrier, he published more than 170 scientific article and also got several scientific awards such as young investigator award of Society of Chemical Engineers, Japan, publication awards of Society for Bioscience and Bioengineering, Japan and Japanese Society for Alternatives to Animal Experiments. He is an AIMBE fellow from 2012 based on such activities. His current research topics are large-scale propagation/differentiation of human iPS cells toward pancreatic beta an hepatic lineages and also engineering of 3D tissues/organs for clinical applications and cell-based assays.
Abstract:
Oxygen transfer-based design of 3D scaffolds for large metabolic tissues: an integrative methodology based on a branching/joining flow channel network and micro-tissue assembly
In large liver tissue engineering, ensuring good mass transfers of oxygen, nutrients or metabolic wastes between the cells and culture medium or blood is the most fundamental issue, but attentions are not so seriously paid when compared with 3D cellular organization or biological optimizations. To ensure oxygen transfer from micro- to macro-scales, we proposed a mew methodology integrating 3D fabrication-based “Top down approach” giving a special scaffold having a branching/joining flow channel with a small chamber which are further filled with various micro-tissue in a random manner as an ”Bottom-up approach”. On the basis of this concept, we fabricated a special 3D scaffold (culture chamber volume 11.63 cm3) by selective laser sintering (SLS) process using poly-?-caprolactone powders. Perfusion culture of model micro-tissue elements, liver cell aggregates, demonstrated the high possibility of scaling up to a clinically relevant size. We also designed and fabricated Raschig ring-like hollow and macroporous micro-scaffolds (1.1 mm in diameter and 1.5 mm in height) to have better stability of the shapes during culture. Perfusion culture of randomly-packed such micro-scaffolds showed better cell growth and functions with very high cell density of the order of 107 cell/cm3. These integrative improvements in terms of oxygen supply in different scales show a promise to engineer large liver tissues through the integration of different approaches according to the scales.
Professor
Department of Chemical System Engineering and Department of Bioengineering, Graduate School of Engineering, University of Tokyo
Room 5A02, Eng. Build. No 3, Hongo 7-3-1, City of Bunkyo, Tokyo 113-8656, Japan
TEL/FAX, +81-3-5841-7073; E-MAIL, sakaiyasu@chemsys.t.u-tokyo.ac.jp
International Research Center on Integrative Biomedical Systems (CIBiS)
Institute of Industrial Science, University of Tokyo, Japan
http://orgbiosys.t.u-tokyo.ac.jp/sakai/index.php?lang=en&page=top
Biography:
Dr. Sakai is the current president of JSAAE (2017-2018) and a professor at Department of Chemical System Engineering, Graduate School of Engineering, University of Tokyo (UTokyo), Japan. He also belongs to Department of Bioengineering. He received Ph.D. in chemical engineering from UTokyo in 1993 and stated his work at Institute of Industrial Science (IIS), UTokyo. In 1997-1998, he stayed in University of Rochester as a visiting scientist. In 2003-2008, he worked as an associate professor of Regenerative Medical Engineering Laboratory, Graduate School of Medicine, UTokyo. He returned to IIS as a professor and then moved to the current position in 2015. During his research carrier, he published more than 170 scientific article and also got several scientific awards such as young investigator award of Society of Chemical Engineers, Japan, publication awards of Society for Bioscience and Bioengineering, Japan and Japanese Society for Alternatives to Animal Experiments. He is an AIMBE fellow from 2012 based on such activities. His current research topics are large-scale propagation/differentiation of human iPS cells toward pancreatic beta an hepatic lineages and also engineering of 3D tissues/organs for clinical applications and cell-based assays.
Abstract:
Oxygen transfer-based design of 3D scaffolds for large metabolic tissues: an integrative methodology based on a branching/joining flow channel network and micro-tissue assembly
In large liver tissue engineering, ensuring good mass transfers of oxygen, nutrients or metabolic wastes between the cells and culture medium or blood is the most fundamental issue, but attentions are not so seriously paid when compared with 3D cellular organization or biological optimizations. To ensure oxygen transfer from micro- to macro-scales, we proposed a mew methodology integrating 3D fabrication-based “Top down approach” giving a special scaffold having a branching/joining flow channel with a small chamber which are further filled with various micro-tissue in a random manner as an ”Bottom-up approach”. On the basis of this concept, we fabricated a special 3D scaffold (culture chamber volume 11.63 cm3) by selective laser sintering (SLS) process using poly-?-caprolactone powders. Perfusion culture of model micro-tissue elements, liver cell aggregates, demonstrated the high possibility of scaling up to a clinically relevant size. We also designed and fabricated Raschig ring-like hollow and macroporous micro-scaffolds (1.1 mm in diameter and 1.5 mm in height) to have better stability of the shapes during culture. Perfusion culture of randomly-packed such micro-scaffolds showed better cell growth and functions with very high cell density of the order of 107 cell/cm3. These integrative improvements in terms of oxygen supply in different scales show a promise to engineer large liver tissues through the integration of different approaches according to the scales.